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Scott Hogan vs Liam Moore
The 24 year old has been in superb form this season, scoring six goals in nine games for the Bees including a hat-trick against Preston in their 5-0 win two weeks ago.
This has been remarkable turn of fortune for Hogan, as the forward has only played 17 games for Brentford following a horrible injury that kept him out for the whole of the 2014/15 season and most of last season.
Looking to make sure that Hogan does not keep up his remarkable run will be Liam Moore. The centre back once again put in brilliant performance at the weekend, as the Royals beat Huddersfield 1-0.
Moore will have to be at his best once again on Tuesday night, as Hogan has the ability to finish when given time and space. It will be therefore important that Moore closes Hogan down at all times, restricting the player's movement.
If Moore can contain Hogan and stop him from getting shots away, then this will be a big plus for Reading as they will be nullifying one of Brentford's main threats.
Nico Yennaris vs John Swift
With Ryan Woods suspended, more emphasis will be put on the role of English born Cypriot centre midfielder Nico Yennaris, who came through the Arsenal academy.
He has featured in all but one of Brentford's league games this season, scoring on the opening day against Huddersfield but not since. The 23 year old also assisted Sullay Kaikai's goal against Wolves in the weekend.
But playing in defensive midfield, being an attacking threat is certainly not top of Yennaris' checklist, instead he will have the tough job of stopping John Swift tonight.
The former Chelsea man has been the Royals' most influential player this season despite a spell on the sidelines, grabbing four goals so far and having a hand in most other threatening moves the Royals make going forward.
The 21 year old of course spent last season on loan at Griffin Park, where he split the opinion of fans as he netted seven goals and got four assists across the course of the campaign. It will be interesting to see what kind of reception he gets from the home supporters tonight.
Harlee Dean vs Yann Kermorgant
The 25 year old centre defender is one of the clubs longest serving players currently playing for The Bees, having been at the club since the 2012/13 season.
Looking to give him problems will be Yann Kermorgant. The Frenchman put in a man of the match display against Huddersfield on Saturday and will be looking to repeat his performance when Reading play Brentford.
Once again Kermorgant is likely to be deployed as the lone striker, so the main focus will be a fascinating one-on-one duel between Dean and Kermorgant. With both players being over six foot tall and ready for a tough battle for 90 minutes.